Friday, February 8, 2008

Major Project! What a Thing!

Phew!

Major Project is literally draining all the juices out of me! I feel
so tired after each meeting as we had to plan out the Literature
Review, Discussion of our Results & Observations and finally come
out with a logical (and of course practical) suggestions to our "Company"
(a major healthcare authority).

Just for your information, Major Project (a.k.a Final Year Project) is
a major component in every Diploma in Temasek Polytechnic. Final year
students are given a selection of topics for them to play with (I mean research on...)

Which means that we poor souls have to conduct strenous research into
their selected fields (food science, nutrition or food service). Once that is
done, students are to report on their findings in an aptly named Final Report.

The finale ends with a Poster Presentation


Now back to where I left off...


My group is in charge of evaluating the effectiveness of a child care centre
programme right here in dear 'ol Singapore. What we were tasked to do includes:

  • Compiling the passing and failure rate of child care centres which participated in the programme
  • Evaluating the effectiveness of the programme (includes coming up with a questionnaire and interviewing the supervisors and cooks of child care centres)
  • Design a pamphlet as a nutrition guide for parents
  • Writing a report on our findings
  • Present our research to a group of industry veterans

Sounds tough huh? You bet!

Well, that's all I can spare for now...it's back to more research, research and more research!

Tuesday, July 31, 2007

Local Authority Overseeing GM Foods in Singapore

Local Authority Overseeing GM Foods in Singapore

About GMAC
http://www.gmac.gov.sg/

The Genetic Modification Advisory Committee (GMAC), a non-profit, multi-agency advisory committee was set up in April 1999 to oversee and advise on the research and development, production, use, handling and release of Genetically Modified Organisms (GMOs), ensuring that these are done in compliance with international standards. GMAC will continue to develop and approve biosafety guidelines regarding GMOs, as well as facilitate the harmonisation of guidelines with international authorities. GMAC has, since, expanded its role to take on creation and enhancement of public awareness on GMOs and GM-related issues.

The objective of this committee was to ensure public safety while allowing for the commercial use of GMOs and GMO-derived products by companies and research institutions, in compliance with international standards.

Biosafety Guidelines for Agriculture-related GMOs

GMAC also promulgates a number of guidelines for the production and distribution of GM foods.
These guidelines are accompanied by several Appendices:

Appendix 1
Questionaire for Risk Assessment of Genetically Modified Organisms (GMOs)
Appendix 2
Risk Assessment Criteria
Appendix 3
Flow Chart for Evaluation, Approval and Registration of Genetically Modified Organisms (GMOs) Related to Agriculture

Below are the objectives of the guidelines:

OBJECTIVES OF GUIDELINES

1.1 These Guidelines are established to ensure the safe movement and use in Singapore
of agriculture-related GMOs.

1.2 These Guidelines provide a common framework for:(a) assessment of risks of agriculture-related GMOs to human health and the
environment; and

(b) approval mechanisms for their release in Singapore.

1.3 These Guidelines address issues related to food safety based on the concept of
substantial equivalence.


Media

In addition to its role as a regulatory organisation, GMAC conducts surveys and frequently distributes press releases to media to inform the public on the latest news related to genetically modified foods. Also, Committee members of GMAC sometimes contribute to forum articles. Placed below are some of the feature news items:

GMAC, 25 Jan 2007

Survey indicated Singaporeans’ Knowledge And Attitudes Towards Genetically Modification has improved slightly since 2001.

Results of a nation-wide survey found that although less Singaporeans have heard of the term “genetic modification”, those who have, are more informed and hold less misconceptions about the subject matter compared to four years.

In May 2005, the Genetic Modification Advisory Committee (GMAC) commissioned a nation-wide survey to understand Singaporean’s knowledge, attitudes and perceptions of genetic modification technology. Conducted by NUS Consulting, this survey is a follow-up of a similar survey conducted four years ago in May 2001.

Data was collected through interviews of 600 Singaporean adults at public places such as shopping centres, MRT stations, bus interchanges and libraries in different parts of Singapore.
The survey, conducted by NUS Consulting, found that 40% of Singaporeans have heard of the term “genetic modification” compared to 50% in 2001. Amongst the respondents who have heard of the term “genetic modification”, almost one-half understood the terminology and basic concepts, a slight improvement from one-third in 2001.

About 20% of respondents thought that eating genetically modified foods could change a person’s genes and about 34% of respondents thought that the human body cannot digest DNA or genes.

Among those that have heard of the term “genetic modification”, attitudes towards genetically modified foods were favorable. More than two-thirds believe the technology would increase food production and confer benefits to the farmers. Just as many would be willing to buy genetically modified foods if they offer tangible benefits such as better appearance, lower price or improved taste.

Less than 8% of the respondents who have heard of the term “genetic modification” believe that GM foods do not confer any benefits at all.

Most of the people interviewed reported learning about genetically modified foods from media such as newspapers, TV, magazines and radio. Just as in the 2001 survey, Singaporeans strongly believe that the Singapore government can be trusted to ensure that GM foods sold here is safe for consumption. They also place great trust in information from doctors, nutritionists and scientists.

It was also found that males, Singaporeans with higher educational levels and those who access the internet frequently tend to have a higher awareness of genetic modification technology. However, those with higher education tend to believe less in the media. Religious background and income do not make significant differences to attitudes.
Since 2001, GMAC through its Subcommittee on Public Awareness, had established several initiatives as part of its public awareness programme. This included:

(a) publishing a website, http://www.gmac.gov.sg/, featuring frequently asked questions, GM-related guidelines and links to several relevant regional and international educational sites,
(b) organizing public forums;
(c) conducting talks and giving interviews to schools, media and civic organizations; and
(d) producing graphics-intensive, easy-to-read brochures on GMOs and GM Foods, targeted at the laymen.

In response to the results of the survey and recognising the importance of nurturing an informed and educated society who would be able to separate facts from myths, GMAC would continue in its efforts to communicate with the public through existing platforms and programs.

The Straits Times Forum Page, 9 Jun 2004
Health and environment the main concern

WE REFER to Dr Andy Ho's commentary, 'Frankenfoods - we need to know' (ST, May 29), and the letters, 'Labelling GM food not easy' (ST, June 1) and 'Don't duck tough questions about GM food' (ST, June 4), from Mr Alvin Loo Eng Kiat and Mr Daniel Koh Kah Soon respectively.
Labelling does, indeed, provide consumers with the information to make choices. However, as Mr Loo pointed out, the issue is not a simple one. There are two key issues that need to be considered before an effective labelling programme can be implemented, such as which types of foods are to be labelled and the determining of threshold levels. We also need to factor in the requirement that any such programme must be scientifically based so as not to fall afoul of World Trade Organisation rules.

There are also issues related to analysis. As a result of protein and DNA degradation during manufacturing or preparation, very little or no DNA can be detected in products such as purified lecithin (for example, soya lecithin), refined vegetable oil (for example, corn oil), starch derivatives (for example, maltodextrin, glucose syrup, corn starch), hydrolysed plant protein (for example, soya sauce powder) and heat-treated or processed finished products (for example, canned products).

The critical issue is ensuring the safety of these products to human health and the environment. However, as Dr Ho highlighted, labelling does not equate to safety.

The story of monarch butterflies and GM maize has been proven to be unlikely. A series of papers in The Proceedings of the National Academy of Science in 2001, including two by Dr John Losey, have shown that it is very unlikely that monarch butterfly larvae could have been poisoned by maize pollen that had been genetically engineered to contain a natural insecticide. Dr John Losey was one of the key authors from Cornell University who had first published the paper in the journal, Nature, that showed the impact of GM maize pollen on monarch butterflies.
So far, there has been no conclusive evidence that any of the GM foods in the market are unsafe. The Genetic Modification Advisory Committee (GMAC) has studied and evaluated reports on the safety tests and risk assessments of GM foods available in the market here, for example, soya bean, corn and canola oil, and agree that they are safe for consumption.

These safety tests and risk assessments are based on well established and accepted scientific evidence, which include but are not restricted to, tests on dietary exposures, toxicity and allergenicity. GMAC will continue to study and evaluate the safety tests and risk assessments of all foods containing GM organisms (GMOs) before they are released into Singapore.
As for the issue of labelling, it is still being examined (see other letter).

GMAC's primary objective is to ensure public and environmental safety, while allowing for the commercial use of GMO and GMO-derived products by companies and research institutions, in compliance with international standards.

GMAC released, in August 1999, the Singapore Guidelines for the Release of Genetically Modified Organisms. They can be found at http://www.gmac.gov.sg/

AIRANI RAMLI (MS)Secretariat,
Genetic Modification Advisory Committee (GMAC)
for Chairman, GMAC

Straits Times Forum Letter - 5th June 2006
GM labelling regime must be practical


I THANK Dr Ooi Can Seng for his comments in his letter, 'GM foods should be labelled clearly' (ST, May 25).

We would like to point out that the lack of international consensus refers to labelling of GM foods and not safety of GM foods, as mentioned in the first paragraph of his letter.
That aside, Dr Ooi raised an issue which Singapore, like other countries in the world, continues to grapple with. Like many other countries in the world, we believe any labelling regime to be implemented must be practical, scientifically derived and effectively implementable.
The issue of labelling raises a host of issues. Dr Ooi has indicated one of them: detection.
The problem of detecting DNA in processed foods is a very real one.

Proteins and DNA degradation occurs during manufacturing and preparation processes. It has been shown, repeatedly, that with current technology, little or no DNA can be detected in products which have undergone significant processing, such as purified lecithin (for example, soya lecithin), refined vegetable oil (for example, corn oil), starch derivatives (for example, maltodextrin, glucose syrup and corn starch), hydrolysed plant protein (for example, soya sauce powder) and heat-treated or processed finished products (for example, canned products).
Commercially available GM crops have been put through rigorous evaluation to establish their safety for consumption.

These safety tests and risk assessments are based on well-established and accepted scientific evidence, which include but are not restricted to tests on dietary exposures, toxicity and allergenicity.

So far, there has been no conclusive scientific evidence that GM foods now in the market are unsafe.

Singapore, as a net importing country, needs to stay alert to worldwide trends and developments.

While the labelling debate continues internationally, we need to ensure that GM foods commercially available are safe for consumption.

The Genetic Modification Advisory Committee will continue to evaluate safety tests and risk assessments of all foods containing GM organisms before they are released into Singapore.
For more information and related news, visit our website at http://www.gmac.gov.sg/

Airani Ramli (MS)Secretariat,
Genetic Modification Advisory Committee
for Chairman, GMAC

Toxicological Analysis

Toxicological Analysis


Lethal Dose 50
NOEL (no-observed-effect-level)
Acceptable Daily Intake (ADI)
Dose Response Curve

Lethal Dose 50

In toxicology, the LD50 (abbreviation for "Lethal Dose, 50%") or median lethal dose of a toxic substance or radiation is the dose required to kill half the members of a tested population. LD50 figures are frequently used as a general indicator of a substance's acute toxicity. The test was created by J.W. Trevan in 1927[1] but is now being phased out in favor of the Fixed Dose Procedure.


http://en.wikipedia.org/wiki/LD50

Some examples of lethal dose 50:

NaCl 40g/kg
Caffeine 200mg/kg
Tetrodoxin 25 microgram/kg
Botulotoxin 100ng/kg

NOEL (no-observed-effect-level)

The greatest concentration or amount of a substance. found by experiment or observation, that causes no alterations of morphology, functional capacity, growth, development or lifespan of target organisms distinguishable from those observed in normal (control) organisms of the same species and strain under the same defined conditions of exposure.


Derived from: http://www.iupac.org/goldbook/N04209.pdf


Acceptable Daily Intake (ADI)

What is an ADI?


The Acceptable Daily Intake is defined as the amount of a food additive that can be ingested daily in the diet without appreciable risk on the basis of all facts known at the time. "Without appreciable risk" refers to the practical certainty that injury will not result, even after a lifetime of experience.


Who determines the ADI?


Basically, scientific expert committees that advise national and international regulatory authorities. The concept was first introduced in 1957 by the Council of Europe and later the Joint Expert Committee on Food Additives (JECFA) of the United Nations Food and Agricultural Organization and World Health Organization. Since then, many other committees and governments have adopted the ADI concept, including the U.S. Food and Drug Administration.


What is the purpose of an ADI?


The ADI is a practical approach to determining the safety of food additives and is a means of achieving some uniformity of approach in regulatory control. It serves to ensure that the actual human intake of a substance is well below toxic levels.How is the ADI determined?
It is based on a scientific review of all available toxicological data on a specific additive D both observations in humans and tests in animals. Laboratory tests in animals determine the maximum dietary level of the additive that is without demonstrable toxic effects, i.e., the "No Observable Effect Level" (NOEL). This level is then extrapolated to man by dividing the no-effect level by a large factor, often 100. This results in a substantially lower level for man, and thus a large margin of safety.


Why is a safety margin necessary?


For two main reasons. First, the NOEL is determined in animals not humans. It is therefore prudent to adjust for possible differences by assuming that man is more sensitive than the most sensitive test animal. Second, the reliability of toxicity tests is limited by the number of animals tested. Such tests cannot represent the diversity of the human population, subgroups of which may show different sensitivities (e.g., children, the old, the ill). Once again it is prudent to adjust for these differences.


What safety margin is normally tested?


Traditionally a safety factor of 100 has been used, based on a 10-fold factor allowed for each of the above reasons. The 100-fold factor (10x10) is not a constant, however, and may be varied according to the characteristics of the additive, the extent of the toxicology data and the conditions of use.


How exact a value is an ADI?


The ADI is an estimated value based on experimental data determined over a lifetime of exposure in animals and derived with a somewhat arbitrary safety factor. It should be regarded as a biological guide to be applied with flexibility, rather than an absolute unalterable constant.


Is it correct then, than an ADI does not represent a toxic dose?


Yes. The ADI is an acceptable level D as the name implies. It is not a toxic dose because of the large safety factor which has been used. Even levels marginally greater than the ADI do not necessarily reflect toxic levels. They simply lessen the safety factor applied.


Is it acceptable for an individual to exceed the ADI on any given day?


Yes, because all you do is slightly reduce the safety margin. For example, if one day you consume even twice the ADI, all you are doing is reducing the safety margin from 100 to 50 for that single day.


http://www.ific.org/publications/qa/adiqa.cfm



Dose Response Curve



http://www.elmhurst.edu/~chm/onlcourse/chm110/outlines/doserespon.html

A dose-response curve is a simple X-Y graph relating the magnitude of a stressor (e.g. concentration of a pollutant, amount of a drug, temperature, intensity of radiation) to the response of the receptor (e.g. organism under study). The response is usually death (mortality), but other effects (or endpoints) can be studied.


The measured dose (usually in milligrams, micrograms, or grams per kilogram of body-weight) is generally plotted on the X axis and the response is plotted on the Y axis. Commonly, it is the logarithm of the dose that is plotted on the X axis, and in such cases the curve is typically sigmoidal, with the steepest portion in the middle.


The first point along the graph where a response above zero is reached is usually referred to as a threshold-dose. For most beneficial or recreational drugs, the desired effects are found at doses slightly greater than the threshold dose. At higher doses still, undesired side effects appear and grow stronger as the dose increases. The stronger a particular substance is, the steeper this curve will be. In quantitative situations, the Y-axis usually is designated by percentages, which refer to the percentage of users registering a standard response (which is often death, when the 50% mark refers to LD50). Such a curve is referred to as a quantal dose response curve, destinguishing it from a graded dose response curve, where response is continuous.


Problems exist regarding non-linear relationships between dose and response, thresholds reached and 'all-or-nothing' responses. These inconsistencies can challenge the validity of judging causality solely by the strength or presence of a dose-response relationship.

http://en.wikipedia.org/wiki/Dose-response_curve

Analytical Methods for GMO Detection

Analytical Methods for Detection of GMO

Gel Electrophoresis
DNA & protein electrophoresis
Chromatography – HPLC, GLC, TLC
PCR

AA Spectrometry

Gel Electrophoresis

Gel electrophoresis is the separation of deoxyribonucleic acid, ribonucleic acid, and protein through an electric charge. It is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or immuno-blotting for further characterization.

Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be analyzed quantitatively by visualizing the gel with UV light and a gel imaging device. The image is recorded with a computer operated camera, and the intensity of the band or spot of interest is measured and compared against standard or markers loaded on the same gel. The measurement and analysis are mostly done with specialized software.



Gel Electrophoresis Apparatus


DNA & protein electrophoresis

Electrophoresis
Protein electrophoresis is a sensitive analytical form of chromatography that allows the separation of charged molecules in a solution medium under the influence of an electric field. A wide range of molecules may be separated by electrophoresis, including, but not limited to DNA, RNA, and protein molecules.

The degree of separation and rate of molecular migration of mixtures of molecules depends upon the size and shape of the molecules, the respective molecular charges, the strength of the electric field, the type of medium used (e.g., cellulose acetate, starch gels, paper, agarose, polyacrylamide gel, etc.) and the conditions of the medium (e.g., electrolyte concentration, pH, ionic strength, viscosity, temperature, etc.).

Some mediums (also known as support matrices) are porous gels that can also act as a physical sieve for macromolecules.

In general, the medium is mixed with buffers needed to carry the electric charge applied to the system. The medium/buffer matrix is placed in a tray. Samples of molecules to be separated are loaded into wells at one end of the matrix. As electrical current is applied to the tray, the matrix takes on this charge and develops positively and negatively charged ends. As a result, molecules such as DNA and RNA that are negatively charged, are pulled toward the positive end of the gel.


Because molecules have differing shapes, sizes, and charges they are pulled through the matrix at different rates and this, in turn, causes a separation of the molecules. Generally, the smaller and more charged a molecule, the faster the molecule moves through the matrix.

When DNA is subjected to electrophoresis, the DNA is first broken by what are termed restriction enzymes that act to cut the DNA is selected places. After being subjected to restriction enzymes, DNA molecules appear as bands (composed of similar length DNA molecules) in the electrophoresis matrix. Because nucleic acids always carry a negative charge, separation of nucleic acids occurs strictly by molecular size.

Proteins have net charges determined by charged groups of amino acids from which they are constructed. Proteins can also be amphoteric compounds, meaning they can take on a negative or positive charge depending on the surrounding conditions. A protein in one solution might carry a positive charge in a particular medium and thus migrate toward the negative end of the matrix. In another solution, the same protein might carry a negative charge and migrate toward the positive end of the matrix. For each protein there is an isoelectric point related to a pH characteristic for that protein where the protein molecule has no net charge. Thus, by varying pH in the matrix, additional refinements in separation are possible.

The advent of electrophoresis revolutionized the methods of protein analysis. Swedish biochemist Arne Tiselius was awarded the 1948 Nobel Prize in chemistry for his pioneering research in electrophoretic analysis. Tiselius studied the separation of serum proteins in a tube (subsequently named a Tiselius tube) that contained a solution subjected to an electric field.
Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis techniques pioneered in the 1960s provided a powerful means of protein fractionation (separation). Because the protein bands did not always clearly separate (i.e., there was often a great deal of overlap in the protein bands) only small numbers of molecules could be separated. The subsequent development in the 1970s of a two-dimensional electrophoresis technique allowed greater numbers of molecules to be separated.

Two-dimensional electrophoresis is actually the fusion of two separate separation procedures. The first separation (dimension) is achieved by isoelectric focusing (IEF) that separates protein polypeptide chains according to amino acid composition. IEF is based on the fact that proteins will, when subjected to a pH gradient, move to their isoelectric point. The second separation is achieved via SDS slab gel electrophoresis that separates the molecule by molecular size. Instead of broad, overlapping bands, the result of this two-step process is the formation of a two-dimensional pattern of spots, each comprised of a unique protein or protein fragment. These spots are subsequently subjected to staining and further analysis.

Some techniques involve the application of radioactive labels to the proteins. Protein fragments subsequently obtained from radioactively labels proteins may be studied my radiographic measures.

There are many variations on gel electrophoresis with wide-ranging applications. These specialized techniques include Southern, Northern, and Western blotting. Blots are named according to the molecule under study. In Southern blots, DNA is cut with restriction enzymes then probed with radioactive DNA. In Northern blotting, RNA is probed with radioactive DNA or RNA. Western blots target proteins with radioactive or enzymatically tagged antibodies.
Modern electrophoresis techniques now allow the identification of homologous DNA sequences and have become an integral part of research into gene structure, gene expression, and the diagnosis of heritable and autoimmune diseases. Electrophoretic analysis also allows the identification of bacterial and viral strains and is finding increasing acceptance as a powerful forensic tool.

http://www.bookrags.com/Electrophoresis


Chromatography

Principles of Chromatography (General)

Chromatography is method of separating mixtures and identifying their components i.e. it's a separation method that exploits the differences in partitioning behavior of analytes between a mobile phase and a stationary phase to separate components in a mixture. Components of a mixture may be interacting with the stationary phase based on charge (ion-ion-interactions, ion-dipole-interactions), van der Waals' forces, relative solubility or adsorption (hydrophobic interactions, specific affinity). There are two theories of chromatography, the plate and rate theories

TLC (Thin Layer Chromatography)

Thin Layer Chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds [1]. It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose immobilised onto a flat, inert carrier sheet. A liquid phase consisting of the solution to be separated dissolved in an appropriate solvent is drawn through the plate via capillary action, separating the experimental solution. It can be used to determine the pigments a plant contains, to detect pesticides or insecticides in food, in forensics to analyze the dye composition of fibers, or to identify compounds present in a given substance, among other uses. It is a quick, generic method for organic reaction monitoring.

http://en.wikipedia.org/wiki/Thin_layer_chromatography

HPLC (High Performance Liquid Chromatography)

High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry. It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixture by using a variety of chemical interactions between the substance being analyzed (analyte) and the chromatography column.

http://en.wikipedia.org/wiki/HPLC


GLC (Gas Liquid Chromatography)

Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen, and the stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside glass or metal tubing, called a column. The instrument used to perform gas chromatographic separations is called a gas chromatograph (also: aerograph, gas separator).

http://en.wikipedia.org/wiki/Gas_liquid_chromatography#Application

Sunday, July 15, 2007

GM Foods: Benefits & Controversies

GM Products: Benefits and Controversies

Benefits

Crops

Enhanced taste and quality
Reduced maturation time
Increased nutrients, yields, and stress tolerance
Improved resistance to disease, pests, and herbicides
New products and growing techniques

Animals

Increased resistance, productivity, hardiness, and feed efficiency
Better yields of meat, eggs, and milk
Improved animal health and diagnostic methods

Environment

"Friendly" bioherbicides and bioinsecticides
Conservation of soil, water, and energy
Bioprocessing for forestry products
Better natural waste management
More efficient processing

Society

Increased food security for growing population



Controversies

Safety

Potential human health impact: allergens, transfer of antibiotic resistance markers, unknown effects Potential environmental impact: unintended transfer of transgenes through cross-pollination, unknown effects on other organisms (e.g., soil microbes), and loss of flora and fauna biodiversity

Access and Intellectual Property

Domination of world food production by a few companies
Increasing dependence on Industralized nations by developing countries
Biopiracy—foreign exploitation of natural resources

Ethics

Violation of natural organisms' intrinsic values
Tampering with nature by mixing genes among species
Objections to consuming animal genes in plants and vice versa
Stress for animal

Labeling

Not mandatory in some countries (e.g., United States)
Mixing GM crops with non-GM confounds labeling attempts

Society
New advances may be skewed to interests of rich countries


http://www.ornl.gov/sci/techresources/Human_Genome/elsi/gmfood.shtml

Further Explanation on GM Foods

What are Genetically Modified (GM) Foods?

Although "biotechnology" and "genetic modification" commonly are used interchangeably, GM is a special set of technologies that alter the genetic makeup of such living organisms as animals, plants, or bacteria. Biotechnology, a more general term, refers to using living organisms or their components, such as enzymes, to make products that include wine, cheese, beer, and yogurt.
Combining genes from different organisms is known as recombinant DNA technology, and the resulting organism is said to be "genetically modified," "genetically engineered," or "transgenic." GM products (current or in the pipeline) include medicines and vaccines, foods and food ingredients, feeds, and fibers.

Locating genes for important traits—such as those conferring insect resistance or desired nutrients—is one of the most limiting steps in the process. However, genome sequencing and discovery programs for hundreds of different organisms are generating detailed maps along with data-analyzing technologies to understand and use them.

In 2003, about 167 million acres (67.7 million hectares) grown by 7 million farmers in 18 countries were planted with transgenic crops, the principal ones being herbicide- and insecticide-resistant soybeans, corn, cotton, and canola. Other crops grown commercially or field-tested are a sweet potato resistant to a virus that could decimate most of the African harvest, rice with increased iron and vitamins that may alleviate chronic malnutrition in Asian countries, and a variety of plants able to survive weather extremes.

On the horizon are bananas that produce human vaccines against infectious diseases such as hepatitis B; fish that mature more quickly; fruit and nut trees that yield years earlier, and plants that produce new plastics with unique properties.

In 2003, countries that grew 99% of the global transgenic crops were the United States (63%), Argentina (21%), Canada (6%), Brazil (4%), and China (4%), and South Africa (1%). Although growth is expected to plateau in industrialized countries, it is increasing in developing countries. The next decade will see exponential progress in GM product development as researchers gain increasing and unprecedented access to genomic resources that are applicable to organisms beyond the scope of individual projects. Technologies for genetically modifying (GM) foods offer dramatic promise for meeting some areas of greatest challenge for the 21st century. Like all new technologies, they also poses some risks, both known and unknown. Controversies surrounding GM foods and crops commonly focus on human and environmental safety, labeling and consumer choice, intellectual property rights, ethics, food security, poverty reduction, and environmental conservation

http://www.ornl.gov/sci/techresources/Human_Genome/elsi/gmfood.shtml

What is Genetically Modified Foods?

What is Genetic Engineering?

Genetic engineering refers to a set of technologies that are being used to change the genetic makeup of cells and move genes across species boundaries to produce novel organisms. The techniques involve highly sophisticated manipulations of genetic material and other biologically important chemicals.

Genes are the chemical blueprints that determine an organism's traits. Moving genes from one organism to another transfers those traits. Through genetic engineering, organisms are given new combinations of genes—and therefore new combinations of traits—that do not occur in nature and, indeed, cannot be developed by natural means. Such an artificial technology is radically different from traditional plant and animal breeding.

Novel organisms

Nature can produce organisms with new gene combinations through sexual reproduction. A brown cow bred to a yellow cow may produce a calf of a completely new color. But reproductive mechanisms limit the number of new combinations. Cows must breed with other cows (or very near relatives). A breeder who wants a purple cow would be able to breed toward one only if the necessary purple genes were available somewhere in a cow or a near relative to cows. A genetic engineer has no such restriction. If purple genes are available anywhere in nature—in a sea urchin or an iris—those genes could be used in attempts to produce purple cows. This unprecedented ability to shuffle genes means that genetic engineers can concoct gene combinations that would never be found in nature.

New risks

Contrary to the arguments made by some proponents, genetic engineering is far from being a minor extension of existing breeding technologies. It is a radically new technology for altering the traits of living organisms by inserting genetic material that has been manipulated by artificial means. Because of this, genetic engineering may one day encompass the routine addition of novel genes that have been wholly synthesized in the laboratory.
Novel organisms bring novel risks, however, as well as the desired benefits. These risks must be carefully assessed to make sure that all effects—both desired and unintended—are benign. UCS advocates caution, examination of alternatives, and careful case-by-case evaluation of genetic enginering applications within an overall framework that seeks to move agricultural systems of food production toward sustainability.


http://www.ucsusa.org/food_and_environment/genetic_engineering/what-is-genetic-engineering.html